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anti vsv g antibody  (ATCC)


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    Structured Review

    ATCC anti vsv g antibody
    Anti Vsv G Antibody, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 341 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+vsv+g+antibody/I1-Hybridoma/bio_rxiv__64898__2026__05__04__722688-110-21-27
    Average 97 stars, based on 341 article reviews
    anti vsv g antibody - by Bioz Stars, 2026-09
    97/100 stars

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    Incubation:

    Article Title: The conserved QTQTX motif in the SARS-CoV-2 spike protein is dispensable for cleavage and lung cell entry of the emerging variant BA.3.2
    Article Snippet: .. After 1 h of incubation at 37 °C, cells were washed with PBS and further incubated in culture medium supplemented with anti-VSV-G antibody (supernatant from I1-hybridoma cells; ATCC CRL-2700). ..

    Article Title: Computational design of an ultrapotent deltacoronavirus miniprotein inhibitor
    Article Snippet: .. Cells were incubated for 18 to 21 h, washed four times with 5 mL of DMEM, and infected with 300 μL VSV (G*ΔG-luciferase) pseudotyped with VSV G in 10 mL of DMEM for 2 h. Cells were washed four times with DMEM and 400 μL of anti-VSV G antibody (I1-mouse hybridoma supernatant, ATCC CRL-2700) was added to 10 mL of DMEM. ..

    Article Title: A stabilized MERS-CoV spike ferritin nanoparticle vaccine elicits robust and protective neutralizing antibody responses.
    Article Snippet: The cells were incubated at 37 ̊C and 5% CO2 for 20-24 h after which the cells were washed three times with DMEM (Gibco) and infected with VSVG/luc95 for 2 h at 37 ̊C and 5% CO2. .. Next, the cells were washed five times with DMEM and incubated in DMEM supplemented with an anti-VSV-G antibody (I1-mouse hybridoma supernatant diluted 1:25, from CRL-2700, ATCC) at 37 ̊C and 5% CO2. ..

    Article Title: Intranasal administration of broad-spectrum macrocyclic peptide inhibitor protects against SARS-CoV-2 Omicron variants
    Article Snippet: .. Then the cell culture medium was removed and replaced with fresh DMEM containing 10 μg mL −1 of anti-VSV-G antibody (I1-Hybridoma ATCC® CRL-2700TM) after 2 h of incubation. ..

    Article Title: A stabilized MERS-CoV spike ferritin nanoparticle vaccine elicits robust and protective neutralizing antibody responses
    Article Snippet: The cells were incubated at 37 °C and 5% CO 2 for 20–24 h after which the cells were washed three times with DMEM (Gibco) and infected with VSVΔG/luc for 2 h at 37 °C and 5% CO 2 . .. Next, the cells were washed five times with DMEM and incubated in DMEM supplemented with an anti-VSV-G antibody (I1-mouse hybridoma supernatant diluted 1:25, from CRL-2700, ATCC) at 37 °C and 5% CO 2 . ..

    Article Title: Intranasal administration of broad-spectrum macrocyclic peptide inhibitor protects against SARS-CoV-2 Omicron variants.
    Article Snippet: .. Then the cell culture medium was removed and replaced with fresh DMEM containing AR TI CL E IN P RE SS ARTICLE IN PRESS 10 μg mL−1 of anti-VSV-G antibody (I1-Hybridoma ATCC® CRL-2700TM) after 2 hours of incubation. ..

    Infection:

    Article Title: Computational design of an ultrapotent deltacoronavirus miniprotein inhibitor
    Article Snippet: .. Cells were incubated for 18 to 21 h, washed four times with 5 mL of DMEM, and infected with 300 μL VSV (G*ΔG-luciferase) pseudotyped with VSV G in 10 mL of DMEM for 2 h. Cells were washed four times with DMEM and 400 μL of anti-VSV G antibody (I1-mouse hybridoma supernatant, ATCC CRL-2700) was added to 10 mL of DMEM. ..

    Article Title: TMPRSS2-mediated coronavirus spike activation and inhibition
    Article Snippet: .. The next day, cells were infected with VSV (G*ΔG-luciferase) for 2 h, followed by five washes with DMEM before the addition of anti-VSV-G antibody (I1-mouse hybridoma supernatant; ATCC, CRL-2700) and medium. ..

    Cell Culture:

    Article Title: Intranasal administration of broad-spectrum macrocyclic peptide inhibitor protects against SARS-CoV-2 Omicron variants
    Article Snippet: .. Then the cell culture medium was removed and replaced with fresh DMEM containing 10 μg mL −1 of anti-VSV-G antibody (I1-Hybridoma ATCC® CRL-2700TM) after 2 h of incubation. ..

    Article Title: Intranasal administration of broad-spectrum macrocyclic peptide inhibitor protects against SARS-CoV-2 Omicron variants.
    Article Snippet: .. Then the cell culture medium was removed and replaced with fresh DMEM containing AR TI CL E IN P RE SS ARTICLE IN PRESS 10 μg mL−1 of anti-VSV-G antibody (I1-Hybridoma ATCC® CRL-2700TM) after 2 hours of incubation. ..

    Virus:

    Article Title: Q1020R in the spike proteins of MERS-CoV from Arabian camels confers resistance against soluble human DPP4.
    Article Snippet: .. To neutralize any residual input virus containing VSV-G, DMEM medium supplemented with anti-VSV-G antibody (culture supernatant from I1-hybridoma cells; ATCC no. CRL-2700) was then added (of note, the antibody was not added to the medium of cells transfected with VSV-G expression plasmid). ..

    Transfection:

    Article Title: Q1020R in the spike proteins of MERS-CoV from Arabian camels confers resistance against soluble human DPP4.
    Article Snippet: .. To neutralize any residual input virus containing VSV-G, DMEM medium supplemented with anti-VSV-G antibody (culture supernatant from I1-hybridoma cells; ATCC no. CRL-2700) was then added (of note, the antibody was not added to the medium of cells transfected with VSV-G expression plasmid). ..

    Expressing:

    Article Title: Q1020R in the spike proteins of MERS-CoV from Arabian camels confers resistance against soluble human DPP4.
    Article Snippet: .. To neutralize any residual input virus containing VSV-G, DMEM medium supplemented with anti-VSV-G antibody (culture supernatant from I1-hybridoma cells; ATCC no. CRL-2700) was then added (of note, the antibody was not added to the medium of cells transfected with VSV-G expression plasmid). ..

    Plasmid Preparation:

    Article Title: Q1020R in the spike proteins of MERS-CoV from Arabian camels confers resistance against soluble human DPP4.
    Article Snippet: .. To neutralize any residual input virus containing VSV-G, DMEM medium supplemented with anti-VSV-G antibody (culture supernatant from I1-hybridoma cells; ATCC no. CRL-2700) was then added (of note, the antibody was not added to the medium of cells transfected with VSV-G expression plasmid). ..



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    Cellular antiviral host defenses are dependent on LATS1. A and B , plaque assay for viral titers from supernatants of WT and Lats1 −/− MEF cells infected <t>with</t> <t>VSV</t> (MOI 0.1) ( A ) or HSV-1 (MOI 0.4) for 16 h. C , immunoblot analysis ( left panel ) and relative quantification ( right panel ) for VSV encoded glycoprotein, G <t>(VSV-G)</t> in WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) for the indicated times. D , immunoblot analysis ( left panel ) and relative quantification ( right panel ) of HSV-1 encoded immediate early transcription factor, ICP4 (HSV-1 ICP4) expression in WT and Lats1 −/− MEF cells infected with HSV-1 (MOI 0.1) for the indicated times. E and F , fluorescent imaging of WT and Lats1 −/− MEF cells infected with VSV-GFP (MOI 0.1) ( E ) or HSV-1-GFP (MOI 0.1) ( F ) for 24 h. Left to right : bright field, GFP, merged channels. Scale bar = 170 μm. Statistical significance was determined using student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).
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    Image Search Results


    Cellular antiviral host defenses are dependent on LATS1. A and B , plaque assay for viral titers from supernatants of WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) ( A ) or HSV-1 (MOI 0.4) for 16 h. C , immunoblot analysis ( left panel ) and relative quantification ( right panel ) for VSV encoded glycoprotein, G (VSV-G) in WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) for the indicated times. D , immunoblot analysis ( left panel ) and relative quantification ( right panel ) of HSV-1 encoded immediate early transcription factor, ICP4 (HSV-1 ICP4) expression in WT and Lats1 −/− MEF cells infected with HSV-1 (MOI 0.1) for the indicated times. E and F , fluorescent imaging of WT and Lats1 −/− MEF cells infected with VSV-GFP (MOI 0.1) ( E ) or HSV-1-GFP (MOI 0.1) ( F ) for 24 h. Left to right : bright field, GFP, merged channels. Scale bar = 170 μm. Statistical significance was determined using student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

    Journal: The Journal of Biological Chemistry

    Article Title: Cytosolic nucleic acid sensing triggers type I interferon activation via the Hippo kinase LATS1

    doi: 10.1016/j.jbc.2026.111204

    Figure Lengend Snippet: Cellular antiviral host defenses are dependent on LATS1. A and B , plaque assay for viral titers from supernatants of WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) ( A ) or HSV-1 (MOI 0.4) for 16 h. C , immunoblot analysis ( left panel ) and relative quantification ( right panel ) for VSV encoded glycoprotein, G (VSV-G) in WT and Lats1 −/− MEF cells infected with VSV (MOI 0.1) for the indicated times. D , immunoblot analysis ( left panel ) and relative quantification ( right panel ) of HSV-1 encoded immediate early transcription factor, ICP4 (HSV-1 ICP4) expression in WT and Lats1 −/− MEF cells infected with HSV-1 (MOI 0.1) for the indicated times. E and F , fluorescent imaging of WT and Lats1 −/− MEF cells infected with VSV-GFP (MOI 0.1) ( E ) or HSV-1-GFP (MOI 0.1) ( F ) for 24 h. Left to right : bright field, GFP, merged channels. Scale bar = 170 μm. Statistical significance was determined using student’s t test (∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05).

    Article Snippet: Primary antibodies used in this study were FLAG (Sigma Life Sciences); VSV-G, HSV-1 ICP4, HSP90, P65 (Santa Cruz Biotechnology); TBK1, p-TBK1, IRF3, p-IRF3, LATS1, p-LATS1, p-YAP, YAP/TAZ, STING, p-STING, p-P65, β-Actin (Cell Signaling Technology); β-Tubulin, GAPDH, HA (Proteintech); P65 (Santa Cruz Biotechnology).

    Techniques: Plaque Assay, Infection, Western Blot, Quantitative Proteomics, Expressing, Imaging